Review of: Waditim

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The new book brings to the fore a stimulating accoimt of those now ways of microbiological determina tion which lay open an approach to various most important problems irhich hitherto could not bo tackled.

To quote one example Our knowledge of the protem motabotism la admittedly very poor, and in many reapeots not sufficiently supported by analytical data.

That IS to a great extent due to the fact that also the recent methods of I? I trust this book will bo widely circulated not only among bio- chemists and clmical pathologigts but espeemDy also among medical people mterested m research The reluibHity of the methods do- scribed m it has been tested assKfuoualy and repeatedly Rappaport has made a special pomt of giving a detailed and minutely exact rOREWOHD vii descnptacwi of all technical proceduree.

The hquid is then pipetted mto a small pipet os described above When a senes of doterminations is to bo made it is recommended to dvasoWc cog of Liquoid in mi of distSled water Of this solution 0 04 ml aro placed upon a small watchglass.

This procedure is repeated until the weight is constant. The flank IS then covered with a watch glass, and is titrated after otie mmute with thioeulfate to a famt yellow Then starch solution is added and the titration is continued until the solution Is colorless.

A famt pmk color will remain From this stock solution the bottles are filled and each one is carefoUy labeled Since each bottle can only be used for drops it la adnsable to mark the bottles each lime after use.

It is further recommended that they be mounted upon a round rotating stand to facilitate the work. P roc e dure A wmnll amount of whole blood or serum is taken up with an eye- dropper fldiich 18 equipped with a rubber bulb and one drop b al lowed to fell through the liquid from a height of 1 cm It is best to start withm the normal range, which is around 1 for scrum and about 1 0o2 for whole blood The next solution b selected accordmg to the behavior of the drop l.

The sedunenta tion pipets are placed into these tubes when a determination is earned out. Glass tubes fig 36 with a diameter of 1 6 mm.

The blood is then allowed to flow by capillary action into the pipet— which should he m a position as horuontsl as possible — up to the mark 0 Then the blood is allowed to flow mto the citrate tost tube, and mixed well, care bemg token to avoid formation of bubbles.

The sedimentation toet niay be set up immediately, or, at the latest, m the course of the next flvo hours If the sedimentation is set up immediately, the pipet may be used agom without being cleaned be- forehand otherwise it may be used only after thorough cleaning dry mg and renewed moistening with citrate solution For Betting up the reaction, the tube is moistened with a sm a l l drop of glycerm at its lower end Almost the entire length of rubber tubmg about 4 cm.

More than 00 mm. Ejajnplc One ml of CoCb solution prepared aa desenbod above requires From this stock solution the working solution is prepared by diluting 1 0 ml with water to ml in a volumetno flask.

The working solution may also be prepared from the stock eolation described m I by diluting 1 08 ml to ml nith distilled water Procedure IScven Waaeennann tubes are placed in a metal rack 05 mm.

In certain pathologic conditions changes occur which lead to flocculation of scrum proteins In a number of dilutions.

It is important to use only the purest grade of NotCOj, c. The reaction may also bo performed as follows Reageuta 1 1 1 Q NoCl and 2.

PrcparaUon This tube can easily be made from a capillary of con slant djameter Melting point tubes or damaged 0 1 ml pipets are suitable.

With the aid of paper graduated m millimeters a mark is made on the glass at the mm pomt Then the tube la carefully heated m the center over a small flame As soon as the glass begins to melt it 18 bent to form a U with two unequal sidee 6 mm difference , the shorter ode bearing the mark fig 6 Fncedun Rubber tubing is attached to the short aim of the U On a wrnall watch glass contalmng a spot of Liquold powder to prevent clotting a few drops of blood from the finger or the ear lobe are mixed quickly The blood IS sucked up through the long side of the U with the aid of the rubber tubmg until ih exactly reaches the mark.

With the aid of the cell volume hematocrit it is possible to find the corrected values for the sedimentation rate, usmg the chart fig. The tubes ore shaken im NER.

The upper part of the chamber pipet a calibrated for 1 ml In divisions of 0 01 or 0 02 ml The adjoining wider part of tho pipot camos marks ot 1.

After each breath the f unn el is closed and the plasma spread out along the walls of the funnel by turning and tilting In this manner the complete saturation of the plasma with COt approximately 40 mm Hg is accomplished.

The mercury column in the rcadmg capillary must remam mtact The dilute lactic acid with nhich the plasma will react is placed m the fillmg cup of the appamtus.

The rest of the procedure and the calculation are earned out as before. The liberated gases aro adjusted to a known volume The pressure pi of this volume of gas m the chamber is read on a mercury manometer Then the gases are oithor expelled from the chamber or absorbed by suitable reagents and the pressure pi IS read at the some volume os before absorption The partial pressure P of the gas at a given volume equals pi -- ps mm Hg.

From this difference the gas volume at OXl and mm, Hg can be calculated. Corroctions for vapor pressure and capillary attraction can be omitted slnco they are the same for both readings, provided that the temponiture remains constant this is gcaerally the case during the short time mtorval of the determination The barometric presBuro will also remam constant dutmg this penod.

The outer rubber tubmg is filled mth mercury with the aid of a Kma. The bulb of the leveling appiaratus has a volume of ml and a connected through 1 meter of vacuum tubing boiled m vaseline m vacuum to a Y-shaped jomt with 2 stopcocks.

Tbo stopcock a at the upper end of the Y jomt Bcrves to let out any posaible air bubbles which might have entered the tube while fillmg it with mercury The Bide arm is connected through rubber tubmg to the levelmg bulb A stopcock with a 3 mm bore connects the vertical arm to the man omoter and the chamber through c screw-clamped rubber tubmg.

The entire apparatus with tlie open monomotor is mounted on a board This board is attached m vertical poeitlon to the edge of a table near a water tap A hole is cut m the board to permit easy rotation of the extraction chamber A water motor or any motor with rpm con be used for shaking The chamber is shaken by means of a metal rod connected to an occontno wheel of the motor on one Hide and a clamp on the water jacket on the other For cleaning the mercury is removed and the apparatus is filled with cleaning solution then washed thoroughly with water and dried with acetone Finally air la sucked through to remove all the acetone.

This can bo achieved by regulating the position of the levelmg bulb and by closing the stopcock b The stop- cock grease as descnbed on page 28 is used but the stopcocks should not be removed and greased too often- The stopcocks should bo cleaned by wipfag them mth clean Imtfreo towels.

If a leak a present, oir nil! To tho dilute solution drops of capryllo alcohol arc added and tho mixture a well shaken before uso Procedure Dran-ing of the blood separation of the plasma and Its saturation with all colar air ore performed os descnbed for the x olu metric analj'sis.

The apparatus. The plasma is saturated with air of 6. The pressure pi of the eKtmcted gases is read at a volume of 0. After food intake the CX j value rises and the alkali reserve drops, beginning approximately one hour after the meal and la st ing for sev eral hours.

In cases of mcreased ventilation of the lung a decrease in alkali reserv e IB found e. By rawmg tho levcimg bulb the manometer reservoir is filled partly mth mercury to establish a high pressure m tho apparatus.

This blue-green solution turns deep brown when oxygen is added Preparation of the blood sample The blood a taken with a dry or parafinaled syringe and transferred to a test tube contanmig a few Fla H Apparatos for Lceptof mceota free from gas.

The difference in mm Hg multiplied by the factor indicated m table results m the oxviren content m volume per cent. We fotmd that it IS not necessary to apply a correction factor as no absorption liquid IS mtroduced mto the chamber The CO content is obtomed by subtracting 1.

The stopcock of the pipet is closed. If no mercury pump is available the blood gas apparatus can be used as follows The pipet IS connected with rubber tubmg to the glass tube above stopcock a and with stopcock b closed the levelmg bulb is lowered, drawing mercury from a reservoir mto the pipet.

After mixmg the blood gases are liberated by ahakmg for three minutes and the pressure ts read at a volume of 0.

By taismg the levelmg bulb the liquid level is brought to the height of the stopcock capillary to expel all air from the chamber During this process no blood must enter the stopcock capillary, sinoe the octyl alcohol, floating on the surface of the blood prevents the blood from leavmg the chamber Then 0 13 ml of feme cyanide solution plus excess femeyamde to account for the stopcock bore is mtroduced Into the apparatus.

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